What starts out as a fun road trip for the Toy Story gang takes an unexpected turn for the worse when the trip detours to a roadside motel. After one of the toys goes missing, the others find themselves caught up in a mysterious sequence of events that must be solved before they all suffer the same fate in this Toy Story of Terror.

Unlike the main television anime series which is mostly set in modern-day Japan, the new series will be a "sealed room" suspense comedy set 100 years in the future. The story begins when Shinnosuke and the entire Nohara family wake up from cold sleep to find themselves aboard a space ship drifting in space. The anime will depict the various events that take place inside the ship.