Cousins Bo and Luke Duke and their car "General Lee", assisted by Cousin Daisy and Uncle Jesse, have a running battle with the authorities of Hazzard County (Boss Hogg and Sheriff Coltrane), plus a string of ne'er-do-wells often backed by the scheming Hogg.

Cousins, Bo and Luke Duke, with the help of their eye-catching cousin, Daisy and moonshine-running Uncle Jesse, try and save the family farm from being destroyed by Hazzard County's corrupt commissioner, Boss Hogg. Their efforts constantly find the 'Duke Boys' eluding authorities in 'The General Lee', their 1969 orange Dodge Charger that keeps them one step ahead of the dimwitted antics of the small southern town's Sheriff, Roscoe P. Coltrane.

When mischievous teenaged cousins Bo and Luke Duke are arrested, both boys are paroled to the care of their Uncle Jesse in Hazzard, sentenced to a summer of hard work. It's not long before the Duke boys learn of Boss Hogg's plans to foreclose on Uncle Jesse's farm. Together, with help from their cousin Daisy, Bo and Luke vow to save the family's property and its storied history of producing the best moonshine in all of Hazzard.